Le final escarpé de la deuxième étape (207 km) de Tirreno-Adriatico jugée à Pomarance a profité à Zdenek Stybar ! Le Tchèque de la formation Etixx-Quick Step a placé une violente attaque au sommet de l’ultime difficulté pour s’imposer en solitaire avec seulement quelques mètres d’avance sur un peloton réglé par Peter Sagan (Tinkoff) devant Edvald Boasson Hagen (Dimension Data). Grâce au jeu des bonifications, Stybar a endossé le maillot de leader.
Tirreno-Adriatico 2016 – 2ème étape – Top 20
| Rank | Name | Nat. | Team | Age | Result |
| 1 | Zdenek STYBAR | CZE | EQS | 31 | 5:10:03 |
| 2 | Peter SAGAN | SVK | TNK | 26 | +1 |
| 3 | Edvald BOASSON HAGEN | NOR | DDD | 29 | +1 |
| 4 | Simon CLARKE | AUS | CPT | 30 | +1 |
| 5 | Alejandro VALVERDE BELMONTE | ESP | MOV | 36 | +1 |
| 6 | Vincenzo NIBALI | ITA | AST | 32 | +1 |
| 7 | Greg VAN AVERMAET | BEL | BMC | 31 | +1 |
| 8 | Tiesj BENOOT | BEL | LTS | 22 | +1 |
| 9 | Michal KWIATKOWSKI | POL | SKY | 26 | +1 |
| 10 | Gianluca BRAMBILLA | ITA | EQS | 29 | +1 |
| 11 | Wouter POELS | NED | SKY | 29 | +1 |
| 12 | Diego ULISSI | ITA | LAM | 27 | +1 |
| 13 | Adam YATES | GBR | OGE | 24 | +1 |
| 14 | Roman KREUZIGER | CZE | TNK | 30 | +1 |
| 15 | Bauke MOLLEMA | NED | TFS | 30 | +1 |
| 16 | Sergei CHERNETSKI | RUS | KAT | 26 | +1 |
| 17 | Tejay VAN GARDEREN | USA | BMC | 28 | +1 |
| 18 | Daniel MORENO FERNANDEZ | ESP | MOV | 35 | +1 |
| 19 | Rigoberto URAN URAN | COL | CPT | 29 | +1 |
| 20 | Jhoan Esteban CHAVES RUBIO | COL | OGE | 26 | +1 |
Tirreno-Adriatico 2016 – Classement général
| Rank | Name | Nat. | Team | Age | Result |
| 1 | Zdenek STYBAR | Czech Republic | EQS | 31 | 5:33:50 |
| 2 | Greg VAN AVERMAET | Belgium | BMC | 31 | +9 |
| 3 | Tejay VAN GARDEREN | United States | BMC | 28 | +9 |
| 4 | Damiano CARUSO | Italy | BMC | 29 | +9 |
| 5 | Daniel OSS | Italy | BMC | 29 | +9 |
| 6 | Gianluca BRAMBILLA | Italy | EQS | 29 | +11 |
| 7 | Bob JUNGELS | Luxembourg | EQS | 24 | +11 |
| 8 | Peter SAGAN | Slovakia | TNK | 26 | +14 |
| 9 | Thibaut PINOT | France | FDJ | 26 | +18 |
| 10 | Sébastien REICHENBACH | Switzerland | FDJ | 27 | +18 |
| 11 | Roman KREUZIGER | Czech Republic | TNK | 30 | +20 |
| 12 | Oscar GATTO | Italy | TNK | 31 | +20 |
| 13 | Vincenzo NIBALI | Italy | AST | 32 | +24 |
| 14 | Jakob FUGLSANG | Denmark | AST | 31 | +24 |
| 15 | Andriy GRIVKO | Ukraine | AST | 33 | +24 |
| 16 | Michele SCARPONI | Italy | AST | 37 | +24 |
| 17 | Bauke MOLLEMA | Netherlands | TFS | 30 | +26 |
| 18 | Michal KWIATKOWSKI | Poland | SKY | 26 | +30 |
| 19 | Wouter POELS | Netherlands | SKY | 29 | +30 |
| 20 | Peter KENNAUGH | Great Britain | SKY | 27 | +30 |
